We have developed a strategy for grafting a protein-protein interface based on the known crystal structure of a native ligand and receptor proteins in a complex. The key interaction residues at the ligand protein binding interface are transferred onto a scaffold protein so that the mutated scaffold
β¦ LIBER β¦
Design, construction and analysis of new metal binding sites in proteins of known structure.
β Scribed by H.W. Hellinga; V. Zhang; J.P. Caradonna; F.M. Richards
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 78 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0162-0134
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## Abstract The present study demonstrates that both the nature (Zn^II^, Cd^II^ or Hg^II^) and supply of metal ions determine whether zinc fingers fold into the wellβknown, fully loaded structures or alternatively populate a variety of structural states under substoichiometric conditions. Metalβbri