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Design considerations and effects of LNA in PCR primers

✍ Scribed by David Latorra; Khalil Arar; J Michael Hurley

Book ID
117593204
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
536 KB
Volume
17
Category
Article
ISSN
0890-8508

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The specificity and reliability of locked nucleic acid (LNA) substitution at the 3' position of allele-specific PCR (AS-PCR) primers for SNP detection was investigated in direct comparison to DNA primers. Both plasmid and human genomic DNA templates were examined in this study. All possible DNA and