Design and synthesis of novel fluorescently labelled nucleosides

✍ Scribed by Jin Yun Zheng; Xiang Ming Feng; Shu Feng Zhang; Yu Fen Zhao

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: French
• Weight: 103 KB
• Volume: 54
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.1813

✦ Synopsis

Abstract

A series of novel 5′‐labelled uridine with fluorophores: N‐alkylcarbazole, alkyloxynaphthalene and alkylfluorene were synthesized through H‐phosphonate by Arbuzov reaction, and were characterized by using ^1^H NMR, ^31^P NMR and ESI‐MS analysis. The fluorescence spectra of all the compounds in solution and solid powder were described. The experimental results showed that the fluorescence emission intensity of 5′‐labelled uridine is very sensitive to solvents. Copyright © 2010 John Wiley & Sons, Ltd.