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Design and scale up of a process for manganese peroxidase production using the hypersecretory strain Phanerochaete chrysosporium I-1512

✍ Scribed by Isabelle Herpoël; Marcel Asther; Jean-Claude Sigoillot


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
172 KB
Volume
65
Category
Article
ISSN
0006-3592

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✦ Synopsis


Manganese peroxidase (MnP) production was performed in a airlift bioreactor in which Phanerochaete chrysosporium I-1512, an MnP hypersecretory strain, was immobilized on a stainless steel mesh. Production was scaled up from a 2.5-L bench scale to a 100-L bioreactor. The yield of MnP was increased 2-fold and reached 6600 U L -1 . These results indicate the feasibility of MnP production on a medium scale, which promises sufficient MnP availability for its use in pulp bleaching at industrial scale.