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Design and operation of a simple continuous-culture apparatus

✍ Scribed by Mohammed Saeed; Frank J. Moss

Publisher
John Wiley and Sons
Year
1966
Tongue
English
Weight
258 KB
Volume
8
Category
Article
ISSN
0006-3592

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✦ Synopsis

A versa& and simple continuous-culture apparatus is described. With this apparat,us, independent control of limiting growth factors and other nutrilites is possible and the conditions of each experiment are reproducible. I n view of the synchronized speed of the feeder syringes, flow variation troubles are not encountered. The device allows the performance of growth experiments a t different dilut,ion rates simultaneously in a single run which makes the comparison of the results more reliable. The operation of the device has been tested successfully with a st>rtdy of adenine deaminase induction in yeast.

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## Abstract A chemostat in which mammalian cells can be raised in continuous suspension culture is described. It is constructed from commercially available parts. This apparatus has the advantage over earlier models in that the medium can be pumped off free of cells, thus suddenly increasing the ce