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Design and evaluation of new, highly sensitive and specific primers for polymerase chain reaction detection of HIV-1 infected primary lymphocytes

✍ Scribed by Katrien Fransen; Ping Zhong; Hans De Beenhouwer; Geert Carpels; Martine Peeters; Joost Louwagie; Wouter Janssens; Peter Piot; Guido van der Groen


Book ID
115638047
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
285 KB
Volume
8
Category
Article
ISSN
0890-8508

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The polymerase chain reaction (PCR) enables rapid and sensitive detection of VZV and HSV DNA and its efficiency depends mainly on the choice of the primers. Primers should hybridize to conserved DNA sequences within the viral genomes i n order to avoid unreliable amplification due to DNA sequence va