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Derivatization of peptides as quaternary ammonium salts for sensitive detection by ESI-MS

✍ Scribed by Marzena Cydzik; Magdalena Rudowska; Piotr Stefanowicz; Zbigniew Szewczuk

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
391 KB
Volume
17
Category
Article
ISSN
1075-2617

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✦ Synopsis

Abstract

A series of model peptides in the form of quaternary ammonium salts at the N‐terminus was efficiently prepared by the solid‐phase synthesis. Tandem mass spectrometric analysis of the peptide quaternary ammonium derivatives was shown to provide sequence confirmation and enhanced detection. We designed the 2‐(1,4‐diazabicyclo[2.2.2] octylammonium)acetyl quaternary ammonium group which does not suffer from neutral losses during MS/MS experiments. The presented quaternization of 1,4‐diazabicyclo[2.2.2]octane (DABCO) by iodoacetylated peptides is relatively easy and compatible with standard solid‐phase peptide synthesis. This methodology offers a novel sensitive approach to analyze peptides and other compounds. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.

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