Derivative spectrum chromatographic method for the determination of trimethoprim in honey samples using an on-line solid-phase extraction technique

✍ Scribed by Kazuhisa Uchiyama; Mari Kondo; Rika Yokochi; Yuri Takeuchi; Atsushi Yamamoto; Yoshinori Inoue

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 185 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201100112

✦ Synopsis

Abstract

A simple, selective and rapid analytical method for determination of trimethoprim (TMP) in honey samples was developed and validated. This method is based on a SPE technique followed by HPLC with photodiode array detection. After dilution and filtration, aliquots of 500 μL honey samples were directly injected to an on‐line SPE HPLC system. TMP was extracted on an RP SPE column, and separated on a hydrophilic interaction chromatography column during HPLC analysis. At the first detection step, the noise level of the photodiode array data was reduced with two‐dimensional equalizer filtering, and then the smoothed data were subjected to derivative spectrum chromatography. On the second‐derivative chromatogram at 254 nm, the limit of detection and the limit of quantification of TMP in a honey sample were 5 and 10 ng/g, respectively. The proposed method showed high accuracy (60–103%) with adequate sensitivity for TMP monitoring in honey samples.