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Derivation of Human Embryonic Stem Cells from Day-8 Blastocysts Recovered after Three-Step In Vitro Culture

✍ Scribed by Miodrag Stojkovic; Majlinda Lako; Petra Stojkovic; Rebecca Stewart; Stefan Przyborski; Lyle Armstrong; Jerry Evans; Mary Herbert; Louise Hyslop; Sajjad Ahmad; Alison Murdoch; Tom Strachan

Book ID
111845988
Publisher
AlphaMed Press
Year
2004
Tongue
English
Weight
556 KB
Volume
22
Category
Article
ISSN
1066-5099

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## Abstract In this study a three step culture system, 2D‐3D sequential culture __in vitro__ and further implantation __in vivo__ was developed to induce human embryonic stem cells (hESCs) into cartilage like tissues. Five‐day‐old embryoid bodies were plated for chondrogenic induction for 27 days (