Depression of peripheral blood monocyte aryl hydrocarbon hydroxylase activity in patients with liver disease: Possible involvement of macrophage factors
✍ Scribed by Theresa C. Peterson; C. Noel Williams
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 593 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0270-9139
No coin nor oath required. For personal study only.
✦ Synopsis
Aryl hydrocarbon hydroxylase activity was detectable in cultured macrophryte monolayers of peripheral blood d y t e origin. Peripheral blood monocytes irobted from patients with biopsy-confirmed liver diwaae a d healthy volunteers. Macrophage monolayers were p -and incubated at 37OC. After 24 hr, the aryl hydnmsrbon hydroxylsse activity and cellular protein COPCdatrrtiOIL were w y e d on cell homogenates. Tbe mmacyte aryl hydnn?arbon hydroxyleee activity in cultured from normal volunteere was 1.23 f 0.16 (n = 19). The aryl hydrocarbon hydroxylase activity in macrophaee cultures from patients with bio p e y -m M liver dleeaee was 0.48 2 0.06 (n = 20). Thie regHeclerts a d g n i f h n t (61%) decrease in monocyte aryl hydmcarbon hydroxylase compared to contrd.. Tbs e0 patients have established cirrhoeia or early et.ge liver dbeem. The eatablbhed cirrhosis group includes al-sntitrgpain ddciency-associated cirrhosie; primary bUary cirrhaeh alcoholic (Laennec's) cirrho-Ssr; cirrhosis, and hemochromatosis. Early liver diimaue is attributed to methotrexate (Stage m), early atage primary biliary cirrhosis and m-antitrypein &&bey. Oru resalts indicate that the depresdon in momcyte aryl hydrocarbon hydroxylmw activity is &rertsr in patients with established Cirrhosis than early mtage liver dimease. OOU rudh further suggeet that cultured monocytes hma patients with liver dimam spontaneously release aolmbk~ i.cton into the culture medium. Incubation of this mndlaaa, containing macrophage factors, with is0-lateaLsp.tscyteseienifican tly depress hepatocyte aryl hydrooybon hydrorylase activity compared to medium obtaired from cuitures of monocytes from normal vol-unteer&
We p m that cultured monocyte aryl hydrocarbon hydmxyhw activity mimica the hepatic enzyme and r e d h t a the etatus of the h m t i c enzyme in mvere liver diasrre. Comqaently, monocyk aryl hydroarbon hydroxyhe activity m y be a weful noninvasive measure of hepatic enzyme function.