Depletion of Infiltrating Leukocytes and Identification of Normal Fibroblasts prior to the Proliferative Assessment of Rare Event Tumor Cells

Enhanced assessment of DNA/proliferative

Enhanced assessment of DNA/proliferative index by depletion of tumor infiltrating leukocytes prior to monoclonal antibody gated analysis of tumor cell DNA

✍ Norma S. Kenyon; Robert J. Schmittling; Olavi Siiman; Alexander Burshteyn; Wade 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 729 KB

Flow cytometry has become an important tool for the analysis of breast tumors, and assessment of S phase fraction and DNA ploidy are potential indicators of tumor aggression. Due to masking or dilution of infrequent tumor cell events, the presence of normal cell types, such as inflammatory cells and