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Depletion of deoxyribonucleoside triphosphate pools in tumor cells by nitric oxide

✍ Scribed by Béatrice Roy; Olivier Guittet; Claire Beuneu; Geneviève Lemaire; Michel Lepoivre


Book ID
113629059
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
331 KB
Volume
36
Category
Article
ISSN
0891-5849

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A rapid, specific, and sensitive method has been developed for the determination of ribonucleoside and deoxyribonucleoside triphosphates in Novikoff hepatoma cells. A simple three-step procedure was used. Extraction of the biological material with 5% cold trichloroacetic acid (TCA); elimination of T