Depletion of calcium stores contributes to progesterone-induced attenuation of calcium signaling of G protein-coupled receptors
โ Scribed by Katja Gehrig-Burger; Jirina Slaninova; Gerald Gimpl
- Publisher
- Springer
- Year
- 2010
- Tongue
- English
- Weight
- 488 KB
- Volume
- 67
- Category
- Article
- ISSN
- 1420-682X
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๐ SIMILAR VOLUMES
Whole-cell functional assays are often used for high-throughput screening (HTS) of molecular targets such as ion channels and G-protein-coupled receptors. A common method for assaying the activity of these membrane proteins is to measure the change in intracellular calcium concentration upon recepto
We have studied the interaction between P1 and P2 purinoceptors in purified type-1 astrocyte cultures from postnatal days 7-8 rat cerebella using single cell microfluorimetry with fura-2. The stimulation of astrocytes with ATP elicits rapid [Ca 2ฯฉ ] i transients showing an EC 50 value of 7.9 ฯฎ 0.3 ยต