## Abstract With insulin methanolβwater, and the ionβpairing agent, hydrochloric acid and trifluroacetic acid (TFA), the character of the first plateau (FP) on the elution curve of frontal analysis in reversed phase liquid chromatography (RPLC) was investigated by onβline UVβspectrometry and identi
Dependence of Elution Curve and Adsorption Isotherms of Insulin on Composition of Mobile Phase of Frontal Analysis in Reversed Phase Liquid Chromatography
β Scribed by Geng Xin-Du; Regnier Fred E
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 615 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0256-7660
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The solute retention mechanism of reversed-phase liquid chromatography is examined by using newly synthesized hydrophilic porous copolymer beads as a stationary phase. It is shown that when water is used as an eluent, the retention time for solutes such as alcohols, ketones and polyethylene glycols
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Contents 1 Introduction 2 Fundamental Definitions in Liquid-Solid Chromato-3 Oscik's Equation for the Capacity Ratio in LSC with Mixed Mobile Phases graphy (LSC) 3.1 Classical Form of Oscik's Equation 3.2 Determination of Parameters in OScik's Equation 3.3 Modified Form of OScik's Equation