Dependence of 13C NMR Chemical Shifts on Conformations of RNA Nucleosides and Nucleotides

✍ Scribed by Ebrahimi, Mohsen (author);Rossi, Paolo (author);Rogers, Christopher (author);Harbison, Gerard S. (author)

• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 135 KB
• Volume: 150
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.2001.2314

✦ Synopsis

Cross-polarization magic-angle spinning solid-state NMR spectroscopy has been used to investigate the dependence of 13 C sugar chemical shifts on specific conformational parameters of a variety of ribonucleotides and ribonucleosides. Solid-state NMR is a valuable tool for nucleoside and nucleotide structural studies since it provides the means to acquire spectra that correspond to single conformations, as opposed to 13 C solution NMR methods. The distinct effects of sugar puckering on the C1 , C4 , and C5 resonances of C2 endo (S type) and C3 endo (N type) furanoid conformations allow us to separate them into two groups. Further analysis of each group reveals an additional dependence of the C1 and C5 resonances on the glycosidic and C4 -C5 exocyclic torsion angles, respectively. However, it is found that the glycosidic conformation cannot independently be determined from sugar 13 C chemical shift data. The statistical methods of exploratory data analysis and discriminant analysis are used to construct two canonical coordinateslinear combinations of chemical shifts which give the statistically optimal determination of the conformation from the NMR data.