Deoxyribonucleic acid homology among the species of the genusBifidobacteriumisolated from animals

✍ Scribed by V. Scardovi; G. Zani; L. D. Trovatelli

• Book ID: 104761920
• Publisher: Springer
• Year: 1970
• Tongue: English
• Weight: 494 KB
• Volume: 72
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00409030

✦ Synopsis

Representatives of the species of the genus Bi]idobaeterium isolated from bovine rumen, intestinal content of honey bees and pig feces were studied for their deoxyribonucleic acid similarities with DNA-DNA filter hybridization-competition experiments. The species were: B. asteroides, B. indieum and B. coryneforme from honey bees; B. ruminale and B. globosum from rumen; B. suis from pig feces; Mitsuoka's species B. thermophilum and B. pseudolongum. Strains of B. bi/idum, B. in/antis, B. longum and B. breve from human sources were included in some comparative experiments. The group of bacteria investigated is largely heterogeneous: virtually no homology exists among the species from rumen and pig feces; the species or types from bees, although unrelated with rumen and pig types, share with these a significant portion of the genome; the competitor DNA's from human strains did not at all react with auy of the homologous systems. The annealing reaction was carried out under stringent conditions to ensure against mismatching of nucleotide sequences (70~ in Denhardt's reactive mixture). The separation within the genus Bifidobacterium of several specific entities is fully substantiated.