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Denitrification inRhodopseudomonas sphaeroidesf.denitrificans

✍ Scribed by Balaram Kundu; D. J. D. Nicholas

Book ID
104777839
Publisher
Springer
Year
1985
Tongue
English
Weight
626 KB
Volume
141
Category
Article
ISSN
0302-8933

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✦ Synopsis

Rhodopseudomonas sphaeroides f. denitrificans grown photosynthetically with NO 3 under anaerobic conditions accumulated NO 2 in the culture medium. In washed cells succinate, lactate, fumarate, citrate and malate, were effective electron donors for the reduction of NO~, NOs and N20 to N2 gas. Nitrate reductase was inhibited by amytal and potassium thiocyanate. Nitrite reductase activity was severely restricted by potassium cyanide, sodium diethyldithiocarbamate, Amytal and 2-n-heptyl-4-hydroxyquinoline-N-oxide whereas N20 reductase was inhibited by NaN> C2H2 and KCNS. Cells incubated with either KXSNO3 or K15NO2 produced *5N20 and lSN 2. A stoichiometry of 2:1 was recorded for the reduction of either NO 3 or NO 2 to N20 and N2 and for N20 to N2 it was 1 : 1.

πŸ“œ SIMILAR VOLUMES

Inhibition of nitrogenase by nitrite and
Inhibition of nitrogenase by nitrite and nitric oxide inRhodopseudomonas sphaeroidesf. sp.denitrificans
✍ W. P. Michalski; D. J. D. Nicholas πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 490 KB

In cells of Rhodopseudomonas sphaeroides f. sp. denitrifieans nitrite and nitric oxide, the products of denitrification, inhibit activity of nitrogenase enzyme. Ferredoxin-linked COz fixation, with H2 as a reductant, was also inhibited by nitrite and NO in denitrifying cells. EPR spectroscopy of c