Denervation increases a neurite-promoting activity in extracts of skeletal muscle
โ Scribed by Henderson, Christopher E.; Huchet, Monique; Changeux, Jean-Pierre
- Book ID
- 109730559
- Publisher
- Nature Publishing Group
- Year
- 1983
- Tongue
- English
- Weight
- 604 KB
- Volume
- 302
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/302609a0
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Polycation-binding neurite-promoting factor (PNPF) from skeletal muscle of bovine fetuses has been compared to mouse tumor laminin by an immunological approach. Antiserum prepared against PNPF blocked the neurite-promoting activity in a dose-dependent manner. The anti-PNPF antiserum bound to Mr = 20
The slow-twitch soleus muscle of the rat hindlimb was denervated by cutting the sciatic nerve in the midthigh on one side. A sham operation was performed on the contralateral side to provide a control soleus muscle. At 24, 48, 72, and 120 hours after these surgical procedures, the animals were sacri