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Dendritic cells fused with mastocytoma cells elicit therapeutic antitumor immunity

✍ Scribed by Laurence Lespagnard; Pascal Mettens; Anne-Marie Verheyden; Nicole Tasiaux; Kris Thielemans; Sonja van Meirvenne; Anja Geldhof; Patrick De Baetselier; Jacques Urbain; Oberdan Leo; Muriel Moser

Publisher: John Wiley and Sons
Year: 1998
Tongue: French
Weight: 183 KB
Volume: 76
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19980413)76:2<250::aid-ijc13>3.0.co;2-g

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✦ Synopsis

Characterization of the spontaneous immune response that frequently occurs in tumor-bearing animals, as well as immunization using dendritic cells pulsed with tumor antigens, suggests that a limiting factor of the tumor-specific immune response may be a defect in the co-stimulatory signal that is required for optimal activation of T cells. In this work, we describe a new approach to improve the antigenpresenting capacity of tumor cells, which does not require a source of purified tumor-associated antigen. We fused P815 mastocytoma cells with bone marrow-derived dendritic cells. We obtained one hybrid that displayed the phenotypic and functional properties of dendritic cells and expressed mRNA coding for the tumor-associated antigen P815 A/B. Injections of irradiated hybrid cells prevented the growth of preimplanted mastocytoma and induced long-lasting tumor resistance.

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