Dendritic cell responses to self-assembled monolayers of defined chemistries

✍ Scribed by Sucharita P. Shankar; Timothy A. Petrie; Andrés J. García; Julia E. Babensee

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 545 KB
• Volume: 9999A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.32487

✦ Synopsis

Abstract

Biomaterial contact triggers dendritic cell (DC) maturation, to an extent depending on the biomaterial, ultimately enhancing an immune response toward associated antigens, implying a role for biomaterials as adjuvants. Self‐assembled monolayers (SAM) of alkanethiols on titanium/gold‐coated surfaces presenting different chemistries were used to study effects of biomaterial surface chemistry on DC maturation. Although DCs treated with OH, COOH, or NH~2~ SAMs showed modest maturation, those treated with CH~3~ SAMs were least mature, all based on cytospins, allostimulatory capacity, or maturation marker expression. Surprisingly, DCs treated with CH~3~ SAMs secreted highest levels of proinflammatory tumor necrosis factor‐α (TNF‐α) or interleukin‐6 (IL‐6) but were least mature. Secretion of anti‐inflammatory mediators by DCs treated with CH~3~ SAMs was not responsible for mitigating DC maturation under these conditions. Interestingly, elevated levels of apoptotic markers were measured associated with DCs and T cells upon CH~3~ SAMs contact. Since phagocytosis of apoptotic DCs has strong immunosuppressive effects on DCs, more apoptotic DCs on CH~3~ SAMs may account for lower DC maturation. Finally, higher expression of cytotoxic T lymphocyte associated antigen receptor‐4 (CTLA‐4) on T cells may imply a mechanism of T cell inhibition on CH~3~ SAMs. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010