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Denaturation studies of active-site labeled papain using electron paramagnetic resonance and fluorescence spectroscopy

✍ Scribed by Ping, Z.A.; Butterfiel, D.A.

Book ID: 119409624
Publisher: Biophysical Society
Year: 1991
Tongue: English
Weight: 575 KB
Volume: 60
Category: Article
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(91)82091-4

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## Abstract N‐Terminally and internally labeled analogues of the hormones angiotensin (AII, DRVYIHPF) and bradykinin (BK, RPPGFSPFR) were synthesized containing the paramagnetic amino acid 2,2,6,6‐tetramethylpiperidine‐1‐oxyl‐4‐amino‐4‐carboxylic acid (TOAC). TOAC replaced Asp^1^ (TOAC^1^‐AII) and