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Denaturation of mouse satellite and ribosomal DNA during hydroxyapatite thermal chromatography of chromatin

✍ Scribed by Iliya G. Pashev

Book ID
104743502
Publisher
Springer
Year
1977
Tongue
English
Weight
337 KB
Volume
3
Category
Article
ISSN
0301-4851

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✦ Synopsis

Mouse DNA and chromatin were melted on hydroxyapatite and the denaturation profiles of ribosomal and satellite DNAs were followed by hybridization with their complementary RNAs. Neither ribosomal nor bulk DNA had significantly different melting profiles in chromatin as compared to DNA. However, most of satellite DNA eluted at higher temperature from chromatin than from purified DNA. One explanation for the higher melting temperature of mouse satellite DNA in chromatin suggests that the complex between this particular DNA component and at least some proteins in chromatin is more stable than the average DNA-protein interaction.

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## Abstract The DNA helix–coil transition has been studied in the presence of high concentrations of manganese ions (about 10^βˆ’3^__M__), which corresponds to the conditions close to equal stability of the A+T and G+C pairs, at the ionic strengths of 10^βˆ’1^, 10^βˆ’2^, and 1.6 Γ— 10^βˆ’3^__M__ Na^+^. With