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Demonstration of β-glucuronidase in disk electrophoresis by means of 5-bromoindol-3-yl-β-d-glucuronide

✍ Scribed by Kazuo Yoshida; Nobuko Iino; Ikue Koga; Keitaro Kato

Book ID: 102625684
Publisher: Elsevier Science
Year: 1974
Tongue: English
Weight: 344 KB
Volume: 58
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(74)90443-6

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✦ Synopsis

A synthetic barium 5-bromoindol-3-yl-P-n-glucuronate was used as the substrate for demonstration of /3-glucuronidase in disk electrophoresis. The advantages of this substrate is that it affords a precise enzyme localization with no diffusion, and, also, a simple and direct method for demonstration of this enzyme without the need for a coupling reaction.

Barium 5-bromoindol-3-yl-P-n-glucuronate was prepared as follows: Ethyl (5-bromoindol-S-y1-~,~,~-tri-O-auetyl-~-~-glucosid)uronate.

To a solution of ethanol (130 ml) containing 600 mg of KOH was added 77

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