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Demonstration of the enzymatic mechanisms of α-N-acetyl-d-glucosamine-1-phosphodiester N-acetylglucosaminidase (formerly called α-N-acetylglucosaminylphosphodiesterase) and lysosomal α-N-acetylglucosaminidase

✍ Scribed by Ajit Varki; William Sherman; Stuart Kornfeld


Book ID
115705849
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
404 KB
Volume
222
Category
Article
ISSN
0003-9861

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The synthesis of substrates and two assa
✍ Karen Gheesling Mullis; Catherine M. Ketcham 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 974 KB

A method for the synthesis and purification of large quantities of four radiolabeled substrates for quantitation of uncovering enzyme is described. Four substrates, [3H]GlcNAc-alpha-P-Man alpha Me, [3H]GlcNAc-alpha-P-uteroferrin, [3H]GlcNAc alpha-P-Man alpha 1-2Man-O-Me, and [3H]GlcNAc alpha-P-Man9G