Demonstration of endothelin (ET) receptors on cultured rabbit chondrocytes and stimulation of DNA synthesis and calcium influx by ET-1 via its receptors
✍ Scribed by Akihiro Kinoshita; Tomoo Tamura; Chiharu Aoki; Tohru Nakanishi; Shizuo Sobue; Fujio Suzuki; Kojiro Takahashi; Masaharu Takigawa
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 369 KB
- Volume
- 19
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
Abstract
Endothelin (ET) receptors on chondrocytes were demonstrated using cultured rabbit costal chondrocytes. After crosslinking the receptors on the cells with ^125^ I‐ET‐1, two major bands of 43 kDa and 46 kDa were separated by SDS‐PAGE. Scatchard analysis demonstrated two classes of ET receptors with K~d~ values of 1 × 10^−10^ M and 5 × 10^−9^ M. The numbers of high‐ and low‐ affinity receptors were 1 × 10^4^ and 2 × 10^5^ per cell, respectively. The binding of ET‐1 to chondrocytes was increased by treatment with PTH, DBcAMP, TGF‐β1, IL‐1β, RA and EGF. ET‐1 stimulated DNA synthesis in cultured rabbit chondrocytes. ET‐1 also stimulated calcium incorporation through the cell membrane of chondrocytes. These findings indicate that ET‐1 has a physiological effect on chondrocytes via its receptors on the cells.