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Demonstration of a cytochrome P-450-dependent steroid 15β-hydroxylase in Bacillus megaterium

✍ Scribed by Anders Berg; Kjell Carlström; Jan-Åke Gustafsson; Magnus Ingelman-Sundberg

Book ID
118310151
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
554 KB
Volume
66
Category
Article
ISSN
0006-291X

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Cloning sequencing and expression of the
Cloning sequencing and expression of the gene for cytochrome P450meg, the steroid-15β-monooxygenase from Bacillus megaterium ATCC 13368
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A 4.3 kb EcoRI fragment carrying the gene for cytochrome P450meg, the steroid-15 beta-monooxygenase from Bacillus megaterium ATCC 13368, was cloned and completely sequenced. The gene codes for a protein of 410 amino acids and was expressed in Escherichia coli and B. subtilis. Protein extracts from t