Demonstration of 1α,25-dihydroxyvitamin D3 receptor-like molecule in ST 13 and 3T3 L1 preadipocytes and its inhibitory effects on preadipocyte differentiation
✍ Scribed by Mayumi Sato; Akiyoshi Hiragun
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 689 KB
- Volume
- 135
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
The active metabolite of vitamin D3, 1 a,25-dihydroxyvitamin D3 (1,25(OH),D,), inhibited morphologic and enzymatic expression during differentiation of preadipocyte to adipocyte. In the presence of -6.4-20 x M 1 ,25(OH)>Di, the triacylglycerol accumulation was only 50% of that of fully differentiated control cells. High-affinity binding sites for 1,25-dihydroxyvitamin Di were detected in two preadipose cell lines. The 1 ,25(OH)2D3 binding component sediments at 3.3 S in 4-24% (wiv) sucrose gradients prepared in hypertonic buffer. Binding assay revealed that N , , , was 70 fmolimg protein and 90 frnolimg protein, and Kd value was 170 p M and 37 p M in cell lines ST 13 and 3T3 L1, respectively. We also found that differentiated adipocytes did not contain specific receptors for 1 ,25(OH)*D3. 1 ,25(OHI2D3, 1 (OH)D3, 24,25(OH)>D3, and 24(OH)D3 all suppressed differentiation of preadipocytes to adipocytes, and the dose required closely reflected the affinities of the various metabolites and the synthetic derivative for 1 ,25(OH)>D3 receptor. It is suggested that the action of vitamin Di on preadipocyte differentiation may result from a receptor-mediated event