Delta aminolevulinic acid dehydratase amounts in lead-exposed subjects: description of a method correlated with the immunoturbidimetric assay
✍ Scribed by N. Despaux-Pagès; E. Comoy; C. Bohuon; C. Boudène
- Publisher
- Springer-Verlag
- Year
- 1986
- Tongue
- English
- Weight
- 530 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0340-0131
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✦ Synopsis
The measurement of 6-aminolevulinic acid dehydratase (ALA D) activity is a good index of lead exposure Recently, we proposed an immunotubidimetric assay which allows determination of the amount of the enzyme This last test is particularly interesting for workers presenting high blood-lead levels We then studied the effects of different agents (dithiothreitol, heat and zinc ions) in restoring the activity of lead-inhibited ALA D The individual or combined effects of these three agents showed an additive restoration of activity The combination of zinc ions with heat and/ or DTT gave the best activations, which correlated perfectly with ALA D amounts Consequently, the catalytic assay using zinc ions and DTT may be used in routine testing as an indirect measurement of ALA D amount.