Delineation of the breakpoint at 18q21.1 in a cell line (KARPAS1106) derived from mediastinal B-cell lymphoma by fluorescencein situ hybridization with multiple YAC clones
✍ Scribed by Akiko Tamura; Tomoaki Akagi; Naozo Nakazawa; Kei Kashima; Shigeo Nakamura; Abraham Karpas; Gary A. Silverman; Yasuo Morishima; Masafumi Taniwaki; Masao Seto
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- French
- Weight
- 195 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
The breakpoint of the 18q21 translocation of B-cellnon-Hodgkin's lymphoma (NHL) cell line Karpas1106P was delineated by fluorescence in situ hybridization (FISH). Karpas1106P was derived from mediastinal lymphoblastic B-cell lymphoma and exhibited the immunophenotype characteristic of marginal-zone B-cell lymphoma (MZL): smIg ؉ , pan-B antigen ؉ , CD5 ؊ , CD10 ؊ and CD23 ؊ . The original G-banded karyotype showed a complex translocation containing t(X;18;13)(q28;q21;q12.1). Double-color FISH (DCFISH) with whole-chromosome-painting (WCP) probes for chromosomes X, 13 and 18, and 18q-specific yeast artificial chromosome (YAC) clones defined t(X;18;13) as ider(X)t(X;18; 13)(q28;q12.3q21.1;q12.1). The immunoglobulin-heavy-chain (IgH) gene was not involved in the chromosomal translocation as detected by DCFISH with VH and C␥ probes. By using contiguous YAC clones mapped from 18q12.3 to q21.1, we identified a YAC clone y852H2 with its breakpoint at 18q21.1. In Karpas1106P, the distal part of chromosome 18 from the breakpoint (18q21.1-qter) was deleted, showing loss of heterozygosity of this region. In addition, the chromosomal segment 18q21.1 was duplicated and inserted to ider(X)t(X;18; 13) between Xq28 and 13q12.1 with maintaining its original orientation. The DNA sequence of the breakpoint region contained in y852H2 can serve as a candidate locus for further molecular dissection to identify the causative gene of MZL.