Delineation of a minimal region of deletion at 6q16.3 in follicular lymphoma and construction of a bacterial artificial chromosome contig spanning a 6-megabase region of 6q16–q21
✍ Scribed by Laura-Jane Henderson; Ichiro Okamoto; Valia S. Lestou; Olga Ludkovski; Marc Robichaud; Mukesh Chhanabhai; Randy D. Gascoyne; Richard J. Klasa; Joseph M. Connors; Marco A. Marra; Douglas E. Horsman; Wan L. Lam
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 672 KB
- Volume
- 40
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Abstract
Regional deletions of 6q are frequent karyotypic alterations in malignant lymphoma and are associated with an adverse clinical outcome. One such region of recurrent deletion is 6q16−q21; however, the specific genes affected have not been identified. Our objective in this study was to identify cases with deletion of 6q16−q21 in follicular lymphoma and to define a minimal region of deletion. A physical map of 6q16.2−q21 was constructed using map information from both sequence‐based and bacterial artificial chromosome (BAC) fingerprint–based maps. Forty‐three BAC clones spanning a 6‐Mb region of 6q16.2–q21 were identified and obtained from the RP‐11 library. Selected BACs were fluorescence‐labeled and hybridized to a series of 34 follicular lymphomas with a regional 6q deletion detected by G banding. Twenty‐four cases with deletion of the 6q16.3 region were detected. A minimal deletion of 2.3 Mb was defined. Our study has identified a limited region of deletion of 6q16.3 that may implicate four known genes in follicular lymphoma and possibly in other cancers. A BAC contig spanning a 6‐Mb region has been anchored to the 6q16.2–q21 region. This map represents a useful resource for gene identification in this region, not only in lymphoma but also in other neoplasms with 6q alterations. © 2004 Wiley‐Liss, Inc.