𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Deletion of specific protein kinase C subspecies in human melanoma cells

✍ Scribed by Masahiro Oka; Kouji Ogita; Hideya Ando; Tatsuya Horikawa; Kazuhito Hayashibe; Naoaki Saito; Ushio Kikkawa; Masamitsu Ichihashi

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
753 KB
Volume
167
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

657 (N.S., U.K.), Japan It has been shown that tumor-promoting phorbol ester, 12-0-tetradecanoylphorbol-13-acetate (TPA), stimulates the proliferation of normal human melanocytes, whereas it inhibits the growth of human melanoma cell lines. The expression of protein kinase C (PKC) subspecies, the major intracellular receptors for TPA, was examined in normal melanocytes and the four melanoma cell lines HM3K0, MeWo, HMV-I, and C361. PKC was partially purified and then separated into subspecies by column chromatography on Mono Q and hydroxyapatite successively, and finally subjected to immunoblot analysis using antibodies specific for

πŸ“œ SIMILAR VOLUMES

Proliferation of human melanoma cells is
Proliferation of human melanoma cells is under tight control of protein kinase C alpha
✍ Konstantin Krasagakis; Carsten Lindschau; Sabine Fimmel; JΓΌrgen Eberle; Petra Qu πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 182 KB

## Abstract Exponential proliferation of human melanoma cells has been associated with low levels of protein kinase C (PKC)‐α. The aim of the present study was to investigate the functional relationship between PKC‐α and melanoma cell proliferation. Treatment of human melanoma cells with the select

Selective modulation of specific protein
Selective modulation of specific protein kinase C (PKC) isoforms in primary human megakaryocytic vs. erythroid cells
✍ Marchisio, Marco ;Bertagnolo, Valeria ;Celeghini, Claudio ;Vitale, Marco ;Capita πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 377 KB πŸ‘ 1 views

We have investigated the pattern of expression of classical (␣, ␀I, ␀II, β₯), novel (␦) and atypical () protein kinase C (PKC) isoforms during the course of human hematopoietic differentiation along the closely related megakaryocytic and erythroid lineages. Using in situ immunofluorescence analysis,

Short-chain fatty acids reduce expressio
Short-chain fatty acids reduce expression of specific protein kinase C isoforms in human colonic epithelial cells
✍ Kurt L. Rickard; Peter R. Gibson; Nicholas J. Wilson; John M. Mariadason; Wayne πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 247 KB πŸ‘ 2 views

LIM1215 colon cancer cells were used as a model of human colonic epithelium to examine the effects of butyrate on protein kinase C (PKC) activity and isoform expression. On Western blot analysis, LIM1215 cells express the PKC isoforms ␣, ␀, , , and , but not β₯, , or . Treatment with 2 mM butyrate fo