Deletion of Ip36 in childhood endodermal sinus tumors by two-color fluorescence in situ hybridization: A pediatric oncology group study
✍ Scribed by Elizabeth J. Perlman; Marcus B. Valentine; Constance A. Griffin; A. Thomas Look
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 540 KB
- Volume
- 16
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Childhood endodermal sinus tumors (CESTs) are a unique category of germ cell tumors involving the testis and extragonadal region in children leu than 4 years of age. Recent studies of CEST have shown recurrent cytogenetic abnormalities involving the short arm of chromosome I, most commonly, a deletion of distal Ip. Experience with neuroblastomas has shown that cytogenetic analyses may underestimate the frequency of I p deletion. To determine the frequency of deletion of I p in CEST and to verify that I p is, in fact, deleted and not translocated, we analyzed ten tumors by two-color fluorescence in situ hybridization on single-cell suspensions of interphase nuclei by using a cosmid probe from the PITSLRE kinase (p58) locus (previously mapped t o 1 p36) cohybridized with plasmid probe pUC I .77 (which recognizes the I q heterochromatic region) to determine the copy number of chromosome I. Eight of the ten tumors examined showed evidence of deletion of I p36. Five of the eight tumors exhibited multiple subclones, and all subclones showed deletion of at least one copy of I p36, indicating that the deletion probably occurred before the development of chromosome I aneusomy. We conclude that deletions of the short arm of chromosome I, specifically lp36, do occur in CEST and probably occur at a higher incidence than that found in neuroblastoma Further studies are needed to determine the degree of overlap of the common area of deletion in CEST with that of neuroblastoma and t o determine whether ID deletion in CEST has prognostic significance. Genes Chrornosom Cancer