We compared the capacity of University of Wisconsin (UW) and of sodium-lactobionate-sucrose (SLS) hypothermic preservation solutions to maintain the integrity of the hepatic cytochrome P-450-dependent mono-oxygenase system. Isolated rat hepatocytes were stored for 0, 10, 24, and 48 hours in UW or SL
Deletion of hydroxyethylstarch from University of Wisconsin solution induces cell shrinkage and proteolysis during and after cold storage of rat liver
β Scribed by N Neveux; J De Bandt; C Charrueau; E Savier; J C Chaumeil; L Hannoun; J Giboudeau; L A Cynober
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 209 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0270-9139
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β¦ Synopsis
Methods
which is controversial, was added as oncotic support to pre-Materials AA solutions, glutamine, and adenosine were from Sigma (La Ver-Abbreviations: UW, University of Wisconsin; HES, hydroxyethylstarch; AA, amino acid; pillie `re, France); 3 H-inulin and 14 C-urea were from Du Pont de Ne-BCAA, branched-chain amino acid. mours (Paris, France); raffinose, lactobionic acid, and reduced gluta-From the
π SIMILAR VOLUMES
Preservation of the liver involves a period of cold (0" to 4" C) ischemia; the longer the ischemic period, the greater the injury to the liver. The mechanisms for cold-induced ischemic injury are not known, but it is clear that after preservation the liver has a reduced capacity to regenerate high-e
Adequate flushing for liver donation requires large fluid volumes delivered at a high flow. This can be achieved more effectively with crystalloid solutions than with colloid-based solutions. This study examined the combination of initial histidine-tryptophanketoglutarate solution (HTK) graft flush