The nif cistrons indentified by complementation analysis in the preceding paper (Dixon et al., 1977) were mapped with respect to hisD and to each other other by P1 cotransduction and three-factor reciprocal crosses. The order obrained was hisD nifB nifA (nifL) nifF nifE nifK nifD nifH. Analysis of h
โฆ LIBER โฆ
Deletion mutants of nitrogen fixation in Klebsiella pneumoniae: Mapping of a cluster of nif genes essential for nitrogenase activity
โ Scribed by Shanmugam, K.T.; Loo, Arnold S.; Valentine, R.C.
- Book ID
- 125426894
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 503 KB
- Volume
- 338
- Category
- Article
- ISSN
- 0304-4165
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The glnA, ntrB and ntrC genes of Klebsiella pneumoniae have been cloned, on a 12 kb HindIII fragment, into the plasmid pACYC184. In a coupled in vitro transcription/translation system the resultant plasmid, pGE100, directed synthesis of five polypeptides (molecular weights 73, 53, 51, 39, 36 kd) fro