𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Deletion mapping of the tumor suppressor locus involved in colorectal cancer on chromosome band 8p21

✍ Scribed by Florence Lerebours; Sylviane Olschwang; Bénédicte Thuille; Annette Schmitz; Pierre Fouchet; Pierre Laurent-Puig; Françoise Boman; Jean-François Fléjou; Geneviève Monges; François Paraf; Pierre Bedossa; Jean-Christophe Sabourin; Rémy-Jacques Salmon; Rolland Parc; Gilles Thomas

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
144 KB
Volume
25
Category
Article
ISSN
1045-2257

No coin nor oath required. For personal study only.

✦ Synopsis

Several somatic genetic alterations have been described in colorectal carcinoma (CRC). Recurrent chromosomal deletions have suggested the presence of tumor suppressor genes (TSG) specifically involved in colorectal carcinogenesis. For one of them, two non-overlapping regions have been proposed on the short arm of chromosome 8, encompassing the LPL and NEFL genes. The short arm of chromosome 8 has been extensively studied in colorectal cancer and in other cancer types. Both regions have been reported as candidate loci for a TSG. In order to delineate a reliable region of deletional overlap on chromosome arm 8p in CRC, a series of 365 CRC samples was selected for the absence of microsatellite instability (RER, replication error); tumor and normal matched DNAs were studied for 54 microsatellite polymorphisms distributed on 8p using multiplex-PCR amplification. After purification of tumor nuclei by flow cytometry based on either the abnormal DNA index or the presence of a high expression of cytokeratin, complete allelic losses on 8p were observed in 48% of cases. Measurement of the DNA index showed that 88% of RER tumors were hyperploid. Complete allelic losses of only part of the short arm were observed on 26 occasions. These data allowed us to define a 1 cM interval of common deletion, flanked by the loci D8S1771 and NEFL, where a putative TSG may be localized.

📜 SIMILAR VOLUMES

Determination of a minimal deletion inte
Determination of a minimal deletion interval on chromosome band 8p21 in sporadic prostate cancer
✍ Jennifer I. Swalwell; Cathy D. Vocke; Youfeng Yang; Jonathan R. Walker; Lynette 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 118 KB 👁 1 views

## Abstract Loss of the short arm of chromosome 8 is a common event in prostatic neoplasms. Previous studies indicate that there may be up to three separate tumor suppressor genes on chromosome arm 8p, based on patterns of allelic loss. The responsible gene or genes have yet to be identified. In th

High frequency of deletion on chromosome
High frequency of deletion on chromosome 9p21 may harbor several tumor-suppressor genes in human prostate cancer
✍ Geetha Perinchery; Nikola Bukurov; Koichi Nakajima; James Chang; Long-Cheng Li; 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 293 KB 👁 1 views

Chromosome 9p has been reported to be a critical region of loss in various cancers. Our present study was designed to determine the frequency of deletions at different loci of chromosome 9p in microdissected samples of normal prostatic epithelium and carcinoma from the same patients. For this purpos

Mapping of a candidate colorectal cancer
Mapping of a candidate colorectal cancer tumor-suppressor gene to a 900-kilobase region on the short arm of chromosome 8
✍ James M. Flanagan; Sue Healey; Joanne Young; Vicki Whitehall; Deborah A. Trott; 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 435 KB 👁 1 views

## Abstract Loss of heterozygosity (LOH) on 8p occurs at high frequencies in many tumor types, including colorectal carcinoma (CRC). We previously used microcell‐mediated chromosome transfer (MMCT) into the CRC cell line SW620 to map a ∼7.7‐Mb colorectal cancer–suppressor region (CRCSR) at 8p22–23.

Genetic and epigenetic alterations of th
Genetic and epigenetic alterations of the candidate tumor-suppressor gene MYO18B, on chromosome arm 22q, in colorectal cancer
✍ Tetsuhiro Nakano; Masachika Tani; Michiho Nishioka; Takashi Kohno; Ayaka Otsuka; 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 261 KB

## Abstract Allelic imbalance (AI) on chromosome arm 22q has been detected in 20%–40% of colorectal cancers, suggesting that this chromosome arm has a tumor‐suppressor gene involved in colorectal carcinogenesis. Recently, we isolated a candidate tumor‐suppressor gene, __MYO18B__, at 22q12.1, that i

Detailed deletion mapping of chromosome
Detailed deletion mapping of chromosome arm 3p in breast cancers: A 2-cM region on 3p14.3-21.1 and a 5-cM region on 3p24.3-25.1 commonly deleted in tumors
✍ Satoshi Matsumoto; Fujio Kasumi; Goi Sakamoto; Masahiko Onda; Yusuke Nakamura; M 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 205 KB 👁 2 views

Loss of heterozygosity (LOH) on 3p is frequent in human renal cell carcinomas, lung cancers, and breast cancers. To define the region(s) on 3p that harbor presumptive tumor suppressor gene(s) for breast cancer, we examined 196 primary breast tumors for their patterns of LOH at 22 microsatellite mark