Delayed-type hypersensitivity to allogeneic mouse epidermal cell antigens

✍ Scribed by Norihisa Ishii; Hiroshi Nakajima; Ichiro Aoki; Takayo Ishii; Toyozoh Takahashi; Kenji Okuda

• Book ID: 104746135
• Publisher: Springer-Verlag
• Year: 1986
• Tongue: English
• Weight: 468 KB
• Volume: 23
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00372666

✦ Synopsis

Footpad swelling response was used to measure the alloantigenicity of epidermal cells (ECs) in delayedtype hypersensitivity (DTH). Strong footpad swelling was oberserved 3 h after the challenge, and it continued for 48 h after the challenge. Genetical incompatibility between the recipients and the ECs was required to induce significant footpad swelling. H-2 or non-H-2 incompatibility between mice and ECs in the sensitization phase sufficed to develop significant footpad swelling. Incompatibility caused by point mutation in the A region induced strong responses when B6.C-H-2 bin12 mice were immunized with B6/J ECs, but the disparity in immunoglobulin h (Igh) allotype genes was insufficient. H-Y antigen on ECs could also elicit the DTH response. Semiallogeneic Fl-derived ECs sensitized the parental recipients. The responses were successfully transferred by immune lymph node cells, but not by immune sera. Treatment of these immune lymph node cells with monoclonal antibodies plus complement revealed that the cells responsible for DTH transfer were Lyt-1 + 2-, Ia-T cells.