Delayed-type hypersensitivity induced by antigen pulsed, bone marrow-derived macrophages

✍ Scribed by Patricia L. Mottram; Jacques F. A. P. Miller

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 640 KB
• Volume: 10
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830100302

✦ Synopsis

Abstract

Mouse bone marrow cells, cultured in specifically conditioned medium, were found to be virtually pure macrophages after 7 days. When pulsed with antigen, these cells induced and elicited delayed‐type hypersensitivity (DTH) with the same efficiency and major histocompatibility complex restrictions as antigen‐pulsed peritoneal exudate cells. Both of these macrophage populations had 20% Ia^+^ cells, as measured by complement‐mediated cytotoxicity. When most of the Ia^+^ cells were removed, the remaining antigen‐pulsed macrophages could not elicit DTH in sensitized mice.