Delayed and reduced cell replication and diminishing levels of DNA polymerase-α in regenerating liver of aging mice

✍ Scribed by Michael Fry; John Silber; Lawrence A. Loeb; George M. Martin

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 966 KB
• Volume: 118
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041180302

✦ Synopsis

Kinetics of cell replication was compared in regenerating livers of Mus musculus at ages ranging between 6 and 32 months. Incorporation of [3H]-thymidine into DNA and autoradiographic analysis showed that the maximal extent of DNA replication following partial hepatectomy became delayed with age. Furthermore, the total fraction of parenchymal and nonparenchymal cells in S phase at different intervals during regeneration diminished as mice aged. The specific activity of DNA polymerase-a, the putative replicative enzyme, declined progressively during aging. The specific activity of DNA polymerase-