Dehydrogenase activity monitoring by flow-injection analysis combined with luminescence-based fibre-optic sensors
✍ Scribed by Sabine M. Gautier; Loïc J. Blum; Pierre R. Coulet
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 660 KB
- Volume
- 266
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
Dehydrogenase achwfies can be measured usmg a blolmnmescence-based fibre-optic sensor associated wnth flow-lqlect~on analysis The sensmg element of the fibre-optic sensor cons&s of bactenal blolummescent euxymes covalently bound on a preactwated polyanude membrane Optmmm condlhons for the dehydrogenase-catalysed reachon compatible with the act~ty and stability of the blolummescent system were selected Hnth lactate dehydrogenase as an enzyme model pemttmg the detectlon of 5 IU 1-l The operational stabtity of the blohmunescent enzymes was not unpruned by repeated contact with the substrates and the products of the dehydrogenase reactron