𝔖 Bobbio Scriptorium
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Degradative plasmids

✍ Scribed by Saunders, J. R.


Book ID
109707272
Publisher
Nature Publishing Group
Year
1977
Tongue
English
Weight
132 KB
Volume
269
Category
Article
ISSN
0028-0836

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Shear-induced degradation of plasmid DNA
✍ C. S. Lengsfeld; T. J. Anchordoquy πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 119 KB

The majority of gene therapy clinical trials use plasmid DNA that is susceptible to shear-induced degradation. Many processing steps in the extraction, purification, and preparation of plasmid-based therapeutics can impart significant shear stress that can fracture the phosphodiester backbone of pol