Degradation potential of biological tissues fixed with various fixatives: Anin vitro study

✍ Scribed by Sung, Hsing-Wen ;Hsu, Chin-Sheng ;Wang, Sheng-Ping ;Hsu, Hung-Liang

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 714 KB
• Volume: 35
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199705)35:2<147::aid-jbm2>3.0.co;2-n

✦ Synopsis

The purpose of this study was to investigate the its helical integrity. This made penetration of enzymes into in vitro degradation potential of porcine pericardia fixed with biological tissue easier. Of the multifunctional EC test groups, various aldehyde or epoxy compound (EC) fixatives, using tissues fixed with tetrafunctional EC (EX-521) or trifunctional bacterial collagenase and pronase. The fixatives investigated EC (EX-313) had relatively better resistance to degradation were formaldehyde (FA), glutaraldehyde (GA), monofunc-than those fixed with bifunctional EC (EX-810). The extent tional EC (EX-131), and multifunctional ECs (EX-810, EX-of degradation for the EX-313 or EX-512 EC fixed tissues was 313, and EX-512). Fresh porcine pericardium was used as a similar to that observed for the FA-or GA-fixed tissues. The control. The test samples were well immersed in a 20-U/ results of this study indicated that the biological tissue fixed mL collagenase solution or a 10-U/mL pronase solution and with monofunctional EC (EX-131) cannot resist bacterial colincubated at 37ЊC at pH 7.5 for 24 h. The extent of degradation lagenase or pronase degradation. However, resistance to degof each test sample was determined by measuring its incre-radation of the multifunctional EC (EX-313 or EX-152)-fixed ment in free amino group content and changes in collagen tissues was comparable to that of the aldehyde (FA or GA)structure, denaturation temperature, and tensile stress after fixed tissues. Therefore, of various EC fixatives, the EC with degradation. In general, the extent of tissue degradation with a greater number of functional groups should be chosen for pronase was more notable than with collagenase. As ob-tissue fixation to increase its resistance to enzymatic degradaserved with fresh tissue, the EX-131 EC fixed tissue radically tion. ᭧ 1997 John Wiley &