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Degradation of sulphur nucleotides in cell-free extracts of Thiobacillus ferrooxidans

✍ Scribed by Dr. O. H. Tuovinen; Mr. B. C. Kelley; Prof. Dr. D. J. D. Nicholas


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
286 KB
Volume
16
Category
Article
ISSN
0233-111X

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✦ Synopsis


Abstract

The cell‐free extracts of Thiobacillus ferrooxidans contain hydrolytic enzymes which cleave sulphate from either adenosine 5′‐sulphatophosphate or adenosine 3′‐phosphate 5′‐sulphatophosphate. These sulphatase activities may be retarded by including either phosphates or adenine nucleotides in the incubation mixtures. Adenosine 3′‐phosphate 5′‐sulphatophosphate and its immediate breakdown product adenosine 3′‐phosphate 5′‐phosphate were degraded at slower rates than adenosine 5′‐sulphatophosphate.


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