Degradation of phenol and phenolic compounds byPseudomonas putidaEKII

✍ Scribed by Christel Hinteregger; Raimund Leitner; Michael Loidl; Andreas Ferschl; Franz Streichsbier

• Book ID: 104652578
• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 932 KB
• Volume: 37
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00178180

✦ Synopsis

The phenol-degrading strain Pseudomonas putida EKII was isolated from a soil enrichment culture and utilized phenol up to 10.6 mM (1.0 g" 1 -1) as the sole source of carbon and energy. Furthermore, cresols, chlorophenols, 3,4-dimethylphenol, and 4-chloro-m-cresol were metabolized as sole substrates by phenol-grown resting cells of strain EKII. Under conditions of cell growth, degradation of these xenobiotics was achieved only in co-metabolism with phenol. Phenol hydroxylase activity was detectable in whole cells but not in cell-free extracts. The specificity of the hydroxylating enzyme was found during transformation of cresols and chlorophenols: orthoand meta-substituted phenols were degraded via 3-substituted catechols, while degradation of para-substituted phenols proceeded via 4-substituted catechols. In cell-free extracts of phenol-grown ceils a high level of catechol 2,3-dioxygenase as well as smaller amounts of 2-hydroxymuconic semialdehyde hydrolyase and catechol 1,2-dioxygenase were detected. The ringcleaving enzymes were characterized after partial purification by DEAE-cellulose chromatography.