Degradation of chondroitin 4-sulphate by tri-fluoroacetolysis: isolation of oligosaccharides from the carbohydrate-protein linkage region

✍ Scribed by Alf Gunnarsson; Sigfrid Svensson; Lennart Rodén

• Book ID: 102641059
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 483 KB
• Volume: 133
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(84)85184-8

✦ Synopsis

Oligosaccharides from the linkage-region tetrasaccharide, beta-D-GlcpA-(1----3)-beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D- Xylp, of chondroitin 4-sulphate were isolated after trifluoroacetolysis. The oligosaccharides were purified by ion-exchange chromatography and paper chromatography and subjected to sugar and methylation analysis and g.l.c.-m.s. The recovery of linkage-region oligosaccharides was approximately 45% after trifluoroacetolysis, calculated according to the D-xylose present in the chondroitin 4-sulphate preparation. The following structures were identified: beta-D-Galp-(1----4)-D-Xylp, beta-D-Galp-(1----3)-D-Galp, beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D-Xylp, beta-D-GlcpA-(1----3)-beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D- Xylp.