✦ LIBER ✦
Degradation of chlorosubstituted aromatic compounds byPseudomonassp. strain B13: fate of 3,5-dichlorocatechol
✍ Scribed by Uwe Schwein; Eberhard Schmidt; Hans-Joachim Knackmuss; Walter Reineke
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 658 KB
- Volume
- 150
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
The degradation of 3,5-dichlorocatechol by enzymes of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 was studied. The following compounds were formed from 3,5-dichlorocatechol: trans-2-chloro-4-carboxymethylenebut-2-en-4-olide, cis-2-chloro-4-carboxymethylenebut-2-en-4-olide, and chloroacetylacrylate as the decarboxylation product of 2-chloromaleylacetate. They were identified by chromatographic and spectroscopic methods (UV, MS, PMR). An enzyme activity converting trans-2chloro-4-carboxymethylenebut-2-en-4-olide into the cis-isomer was observed.