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Degradation of carbofuran by an enrichment culture developed from carbofuran-treated Azolla plot

✍ Scribed by Singh, Neera; Sethunathan, N


Book ID
101215591
Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
83 KB
Volume
55
Category
Article
ISSN
1526-498X

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✦ Synopsis


Standing water from carbofuran-treated Azolla plots showing accelerated degradation was further enriched by ®ve repeated transfers to carbofuran-supplemented mineral salts medium. This enrichment culture developed from standing water of carbofuran-treated Azolla plot can utilise carbofuran as sole source of carbon and nitrogen. The enrichment culture was able to hydrolyse nearly 100% of [ring-14 C]carbofuran to carbofuran phenol in ®ve days, which accumulated in the medium, while the carbamate side-chain in [carbonyl-14 C]carbofuran was readily mineralized to [ 14 C]carbon dioxide. Enrichment culture was able to degrade carbofuran up to 1000 mg ml À1 levels in mineral salts medium with ease.


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Suspensions of soil repeatedly treated with carbofuran under glasshouse conditions enhanced the degradation of carbofuran signiÐcantly in a mineral salts medium. Ninety-seven per cent of the applied carbofuran degraded in a medium inoculated with soil suspensions treated with carbofuran, compared to