Degradation of ATP by membrane-bound enzymatic activities in Dictyostelium discoideum monitored by high-pressure liquid chromatography

✍ Scribed by Jessica L. Hodge; Edward F. Rossomando

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 268 KB
• Volume: 102
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90316-4

✦ Synopsis

This report describes the separation of ATP, ADP, AMP, adenosine, adenine, and CAMP using reverse phase high-pressure liquid chromatography on FBondapack C,,. ATP, ADP, AMP, and adenine could be eluted with ammonium phosphate while 9% methanol was required to elute adenosine and CAMP. The fate of ATP following its incubation with plamsa membranes from Dictyostelium discoideum was determined using reverse phase high-pressure liquid chromatography to analyze reaction components. Degradation of ATP was noted, as well as the formation of hydrolytic products such as ADP, AMP, and adenosine. The amount of AMP formed (46.3 nmoV30 min), as determined by separation from reaction components by high-pressure liquid chromatography, corresponds to the amount of AMP formed (55.8 nmol/30 min) as determined when the separation was made by thinlayer chromatography.