Definition of three species-specific monoclonal antibodies recognizing antigenic structures present on human embryonal carcinoma cells which undergo modulation during in vitro differentiation
✍ Scribed by C. Blaineau; F. Connan; D. Arnaud; P. Andrews; L. Williams; J. McIlhinney; P. Avner
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- French
- Weight
- 801 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Three novel species-specific monoclonal antibodies 5.I.H, 8.7.D and 13.7.A raised against semi-purified detergent solubilized fractions of the Tera I embryonal carcinoma (EC) cell line are restricted in their in vitrodistribution to undifferentiated human EC cell lines. Competition experimenb have established that distinct antigenic specificities are seen by the 3 different monoclonal antibodies. All 3 antigens 5.I.H. 8.7.D and 13.7.A. defined by these monoclonal antibodies, undergo developmental regulation and cease to be expressed on Tera 2 clones 5 and I 2 after retinoic-acid-induced differentiation and on UCR LON HT 39/7 Celts after phorbol-ester-induced differentiation. These results taken together with the extremely limited in vivotissue distribution of the defined antigens suggest that the 5. I.H, 8.7.D and 13.7.A monoclonal antibodies define distinct onco-foetal antigens.