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Defining the Orientation of the 15 N Shielding Tensor Using 15 N NMR Relaxation Data for a Protein in Solution

โœ Scribed by Boyd, Jonathan; Redfield, Christina


Book ID
127294797
Publisher
American Chemical Society
Year
1998
Tongue
English
Weight
36 KB
Volume
120
Category
Article
ISSN
0002-7863

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3D NMR Experiments for Measuring15N Rela
โœ Michael Caffrey; Joshua Kaufman; Stephen J. Stahl; Paul T. Wingfield; Angela M. ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 134 KB

A suite of 3D NMR experiments for measuring 15N-ยฟ1Hยฟ NOE, 15N T1, and 15N T1rho values in large proteins, uniformly labeled with 15N and 13C, is presented. These experiments are designed for proteins that exhibit extensive spectral overlap in the 2D 1H-15N HSQC spectrum. The pulse sequences are read