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Defining components required for transport from the ER to the golgi complex in yeast

✍ Scribed by Anna P. Newman; Susan Ferro-Novick

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 848 KB
Volume: 12
Category: Article
ISSN: 0265-9247
DOI: 10.1002/bies.950121006

No coin nor oath required. For personal study only.

✦ Synopsis

Several complementary approaches have been fruitful in the study of transport from the ER to the Golgi complex in yeast. Mutational analysis has led to the identification of genes required for this process, many of which are now being studied at the molecular and biochemical level. In the case of SEC18, DNA sequence analysis has demonstrated homology to a factor needed for transport in mammalian in vitro systems. In addition, the events that take place at this stage of the secretory pathway have been reconstituted in vitro.

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The cellular machinery responsible for conveying proteins between the endoplasmic reticulum and the Golgi is being investigated using genetics and biochemistry. A role for vesicles in mediating protein traffic between the ER and the Golgi has been established by characterizing yeast mutants defectiv